Genetic diversity and differentiation of four landraces of Fritillaria thunbergii
Miq. and two congeners Fritillaria cirrhosa
D. Don and Fritillaria anhuiensis
S. C. Chen et S. F. Yin were evaluated using ISSR markers. The results showed that the genetic diversity of F. thunbergii
was high at the specie level but relatively lower at the landrace level.
A high level of genetic differentiation among four F. thunbergii
landraces was detected based on the gene differentiation coefficient and the AMOVA, in line with the low inter-landrace gene flow. MS-tree analysis showed that the four F. thunbergii
landraces were clustered on adjacent positions of the tree, and that Kuanye (Ft-KY) landrace was relatively distantly related to other landraces. In line with the MS-tree analysis, PCoA revealed that the three species of Fritillaria
can be divided into two groups and three subgroups, among which there occurred a remarkable genetic differentiation.