Abstract

Pollen viability was evaluated by TTC. The stigma receptivity was estimated by benzidine-H2O2.Pollination characteristics and breeding system were checked by investigating the pollinators on the spot, bagging experiment, pollen-ovule ratios (P/O) and observing Pollen morphology with scanning electron microscopy. The results showed that pollen viability of Fritillaria ussuriensis was more than 90% during the period from the second day to the fifth day after flowering. Pollen viability decreased after the sixth day, and 71.3% pollen still kept viability after the tenth day. Stigma receptivity could last about 8 days, and was in the strongest condition on the third and fourth day after blooming, then decreased after the fifth day. The breeding system was facultative autogamy which relied mainly on self-pollination, supplemented by anthophilous cross-pollination. The results provides the theory and technology basis for breed selection, hybridization breeding, introduction cultivation and species conservation of Fritillaria ussuriensis, provides some data in reproductive ecology for further study on evolution and the phylogeny of Fritillaria plants in Liliaceae.